![]() The latter effects were sustained at 72 h R in a subset of pigs ( n = 7). Myocardial infarct size (% AAR) was significantly smaller in AdNOS3 than in control and AdRR5 and associated with a significantly greater reduction in cardiac myeloperoxidase activity, a marker of neutrophil infiltration. At 4 h R, LV systolic (d P/d t max) and diastolic (d P/d t min) function was better preserved in AdNOS3- than in AdRR5-injected pigs (2,539 ± 165 vs. ![]() ![]() Retrograde NOS3 gene transfer selectively increased NOS3 expression and NO bioavailability in the area at risk (AAR) without changing endogenous NOS isoform expression. Hemodynamic and pathological changes were measured in pigs in the absence ( n = 11) or presence of prior intracoronary retroinfusion of human NOS3 (AdNOS3, 5 × 10 10 PFU, n = 13) or control vector (AdRR5, 5 × 10 10 PFU, n = 11). I/R injury was induced by balloon occlusion of the left anterior descending artery for 45 min followed by 4 or 72 h reperfusion. We investigated whether cardioselective nitric oxide synthase 3 (NOS3) gene transfer could confer myocardial protection against I/R injury in pigs and examined potential molecular mechanisms. Nitric oxide modulates the severity of myocardial ischemia–reperfusion (I/R) injury.
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June 2023
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